The Core Laboratory Facility's new Zeiss LSM 710 confocal microscope has excitation laser lines at 405, 458, 488, 514, 561 and 633nm. It is has three separate spectral detection channels and is
capable of detecting many standard fluorophores, including DAPI, CFP, GFP, fluorescein, Rhodamine, Texas Red, RFP, CY5 and several of the AlexFluor dyes. Suitable applications include imaging of
single, double and triple-labeled specimens, 3-dimensional image analysis, colocalization studies, immunofluorescence, time series and FRAP experiments. A separate workstation with a copy of the
Zeiss LSM software is available for image analysis and processing
Faculty and students who wish to use the microscope should follow the instructions in the New Users Guide.
Prepared slides will be used during the training session in order to acquaint new users with all aspects of the microscope, however, new users are also encouraged to bring their own samples to the training session if any are available. It is also highly recommended that new users review the UMD Zeiss LSM710 Quickstart Guide before training begins.
The rate schedule applies to different users and is current for the 2013/2014 academic year. Fees are used to help cover the cost of the service contract on the microscope, which is partially subsidized by the department, and to help pay for materials such as lens paper, lens cleaning solution and mercury bulbs.
The microscope is available to trained users on an equal basis. An individual user may reserve one three hour block of time during peak hours and an additional 6 hours of time during off-peak hours. Scheduling is accomplished using Google Calendar. For information on how to apply for and schedule instrument time using Google Calendar, please click here.
All users are encouraged to familiarize themselves with the complete list of policies governing the use of the LSM710.
The facility manager is available during normal business hours to provide technical assistance to users. Additional resources include the following Imaging Core protocols:
|Microscope||Axiovert Observer Z1 (inverted) with universal stage insert available|
|Conventional Fluorescence Filters||Rhodamine, Fluorescein, LP505|
|Lasers||405 diode, Argon (458, 488, 514 nm), 561nm and HeNe 633nm|
|Objective Lenses||10x/0.3, 40x/1.3 Oil DIC Plan NeoFluar, 63x/1.4 Oil DIC Plan Apo, 63x/1.2 W Korr C-Apo, 100x/1.4 Oil DIC Plan Apo|
|Detectors||Three spectral detections channels plus transmitted light PMT|
||MT/Phy/FRET/FRAP AIM-ZEN 2009 package|
Imaging Core Facility
0107 Microbiology Building
University of Maryland