• BISI - Molecular & Cellular Biology (MOCB)

Host-pathogen interactions, molecular mechanisms of pathogenesis for Pseudomonas aeruginosa, allosteric regulation of molecular complexes, bacterial signal transduction and small molecular inhibitors.

Kim, S.K., Lormand, J.D., Weiss, C.A., Eger, K.A., Turdiev, H., Turdiev, A., Winkler, W.C., Sondermann, H., Lee, V.T. 2019. A dedicated diribonucleotidase resolves a key bottleneck for the terminal step of RNA degradation. Elife. Jun 21;8. pii: e46313. PMID: 31225796

Orr, M.W., Weiss, C.A., Severin, G.B., Turdiev, H., Kim, S.-K., Liu, K., Tu, B.P., Waters, C.M., Winkler, W.C., Lee V.T. 2018. A subset of exoribonucleases serve as degradative enzymes for pGpG in c-di-GMP signaling. J. Bacteriol. pii: JB.00300-18. PMID: 30249708

Cole, S.J., Hall, C.L., Schniederberend, M., Farrow III, J.M., Goodson, J.R., Pesci, E.C., Kazmierczak, B.I., Lee V.T. 2018. Host suppression of quorum sensing during catheter associated urinary tract infections. Nature Comm. 9(1):4436. PMID: 30361690

Zhou E., Seminara, A.B., Kim, S.K., Hall, C.L., Wang Y, Lee V.T. 2017. Thiol-benzo-triazolo-quinazolinone Inhibits Alg44 Binding to c-di-GMP and Reduces Alginate Production by Pseudomonas aeruginosa. ACS Chem Biol. 12(12):3076-3085. doi: 10.1021/acschembio.7b00826. PMID: 29091392

Roelofs, K.G., Jones, C.J., Helman, S.H., Shang, X., Orr, M.W., Goodson, J.R., Galperin, M.Y., Yildiz, F.H., and Lee, V.T. 2015. Systematic identification of cyclic-di-GMP binding proteins in Vibrio cholerae reveals a novel class of cyclic-di-GMP-binding ATPases associated with Type II secretion systems. PLoS Pathogens. 11(10):e1005232. PMID: 26506097

Orr, M.W., Donaldson, G.P., Severin, G.B., Wang, J., Sintim, H.O., Waters, C.M., and Lee, V.T. 2015. Oligoribonuclease is the primary degradative enzyme for pGpG in Pseudomonas aeruginosa that is required for cyclic-di-GMP turnover. Proc Natl Acad Sci U S A. 8;112(36):E5048-57. PMID: 26305945

Cole, S.J., Records, A.R., Orr, M.W., Linden, S.B., and Lee, V.T. 2014. Catheter-associated urinary tract infection by Pseudomonas aeruginosa is mediated by exopolysaccharide-independent biofilms. Infect Immun. 82(5):2048-58. doi: 10.1128/IAI.01652-14. PMID: 24595142

Lieberman, O.J., Orr, M.W., Wang, Y., and Lee, V.T. 2014. High-throughput screening using the differential radial capillary action of ligand assay identifies ebselen as an inhibitor of diguanylate cyclases. ACS Chemical Biology. 9(1):183-92. doi: 10.1021/cb400485k. PMID: 24134695

Corrigan, R.M., Campeotto, I., Jeganathan, T., Roelofs, K.G., Lee, V.T. and Gründling, A. 2013. Systematic identification of conserved bacterial c-di-AMP receptor proteins. Proc Natl Acad Sci U S A. 110(22):9084-9. PMID: 23671116 

Donaldson, G.P., Roelofs, K.G., Luo, Y., Sintim, H.O., Lee, V.T. 2011. A Rapid Assay for Affinity and Kinetics of Molecular Interactions with Nucleic Acids. Nucleic Acids Res. 40(7): e48. doi: 10.1093/nar/gkr1299. PMID: 22210888

Roelofs, K.G., Wang, J., Sintim, H.O., Lee, V.T. 2011. Differential radial capillary action of ligand assay for high-throughput detection of metabolite interactomes. Proc Natl Acad Sci U S A. 108(37):15528-33. PMID: 21876132 

Merighi, M., Lee, V.T., Hyodo, M., Hayakawa, Y., and Lory, S. 2007. The second messenger bis-(3’-5’)-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Mol. Microbiol. 65: 876–895. PMID: 17645452

Lee, V.T., Matewish, J.M., Kessler, J.L. Hyodo, M., Hayakawa, Y., and Lory, S. 2007. A Cyclic-di-GMP Receptor Required for Bacterial Exopolysaccharide Production. Mol. Microbiol. 65: 1474-1484. PMID: 17824927 

Ph.D. - University of California - Los Angeles, 2000