Antony M Jose
Office Phone: 301.405.7028
Lab Phone: 301.405.9513
Lab: 2205 Bioscience Research Building
Office Address: 2136 Bioscience Research Building
Graduate Program Affiliations
Deciphering the cell code that perpetuates animal life
The information required to perpetuate animal life must be contained even within a single cell that begins each generation (e.g. fertilized egg in sexually reproducing animals). The DNA within
this cell encodes what can be made but does not determine what is made. What is made depends on how the cell interprets the DNA sequence, and requires instructions and machinery in addition to
the DNA. Thus, life 'begins' with two distinct forms of information: the linear DNA sequence that is faithfully replicated during cell divisions, and a three-dimensional arrangement of molecules
that dictate what is made using DNA, that can change during development, and that returns to a similar configuration at the start of each generation. These two interdependent stores of
information – one replicating with every cell division and the other cycling with a period of one generation – coevolve while perpetuating an organism and together define the cell
Understanding the cell code and how it is propagated during development such that it is recreated in a similar configuration at the start of each generation has implications for evolution, origins of inherited diseases, and consequences of genome engineering. Taking advantage of our recent ability to induce transgenerational epigenetic inheritance, i.e. modify non-genetic aspects of the cell code, our goal is to use reductionist, systems, and engineering approaches to understand the information required to build and perpetuate an animal.
Devanapally S, Allgood S, Jose AM. Mating can cause transgenerational gene silencing in Caenorhabditis elegans. BioRxiv. Online Jun 11.
Jose AM. Replicating and cycling stores of information perpetuate life. BioRxiv. Online Aug 26.
Raman P, Zaghab S, Traver EC, Jose AM. The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Nucleic Acids Research. 45(14):8463-73.
Choi YS, Edwards LO, DiBello A, Jose AM. Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs. Nucleic Acids Research. 45(10):e87.
Marré J and Jose A. Inheritance of extracellular nutrition and information in Caenorhabditis elegans. Molecular Reproduction & Development. 84(4):283.
Marré JA, Traver EC, Jose AM. Extracellular RNA is transported from one generation to the next in C. elegans. Proceedings of the National Academy of Sciences USA. 113(44):12496-501.
Le HH, Strauss B, Bloodgood M, Jose AM. Tissue homogeneity requires inhibition of unequal gene silencing during development. Journal of Cell Biology. 214(3): 319-331.
Blumenfeld AL, Jose AM. Reproducible features of small RNAs in C. elegans reveal NU RNAs and provide insights into 22G RNAs and 26G RNAs. RNA. 22:184-192.
Jose AM. Movement of regulatory RNA between animal cells. genesis. 53(7): 395-416.
Devanapally S, Ravikumar S, Jose AM. Double-stranded RNA made in C. elegans neurons can enter the germline and cause transgenerational gene silencing. Proceedings of the National Academy of Sciences USA. 112(7):2133-8.