Wade Winkler headshot
Contact Info
Office: 3115 Bioscience Research Bldg
Lab: 3112 Bioscience Research Bldg
Office Phone: 301-405-7780
Lab Phone: 301-405-9937
Wade Winkler
Professor

Teaching

  • Principles of Microbiology (BSCI283)
  • Microbial Genetics (BSCI412)
  • Teaching Science and Professional Development (BISI701)

Graduate Program Affiliations

  • BISI-Molecular & Cellular Biology (MOCB)

Research Interests

All organisms regulate the expression of their genes. These genetic regulatory processes are often responsive to specific chemical or physical cues. The overarching goal of my laboratory is to discover new classes of genetic regulatory elements and reveal their underlying molecular mechanisms, including their integration of physiological signals. More specifically, we investigate bacterial regulatory RNAs and the proteins that associate with them. Over the years, my lab has investigated many different riboswitches that respond to a range of cellular signals, including uncharged tRNAs, metabolites, metal ions, and RNA-binding proteins. My lab has also discovered novel small RNAs and long 5' leader regions in Bacillus subtilis and other Gram-positive bacteria. In addition, we study RNA degradation pathways. Together, these research endeavors provide key information about the biology of bacterial pathogens and will continue to inspire the development of new synthetic biology tools. 


Education

  • Ph.D., The Ohio State University, 2002
  • Postdoctoral Training, Yale University, 2004

All Publications

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Latest Publications

Jermain MD,Tran T,Jenkins CC,Nasisi BH,Winkler WC
mBio. 2025 Dec 10;16(12):e0142925. doi: 10.1128/mbio.01429-25. Epub 2025 Oct 28
Myers TM,Burnim AA,Jermain MD,Sondermann H,Lee VT,Jiang X,Winkler WC
Nucleic Acids Res. 2025 Nov 26;53(22):. doi: 10.1093/nar/gkaf1384. Epub
Mortensen S,Kuncová S,Lormand JD,Myers TM,Kim SK,Lee VT,Winkler WC,Sondermann H
Nucleic Acids Res. 2025 Jan 7;53(1):. doi: 10.1093/nar/gkae1235. Epub
Kim SK,Orr MW,Turdiev H,Jenkins CC,Lormand JD,Myers TM,Burnim AA,Carter JA,Kung WC,Jiang X,Sondermann H,Winkler WC,Lee VT
Cell Rep. 2024 Sep 24;43(9):114759. doi: 10.1016/j.celrep.2024.114759. Epub 2024 Sep 13
Elghondakly A,Jermain MD,Winkler WC,Ferré-D'Amaré AR
Structure. 2024 Sep 5;32(9):1488-1497.e5. doi: 10.1016/j.str.2024.06.001. Epub 2024 Jul 2
Myers TM,Ingle S,Weiss CA,Sondermann H,Lee VT,Bechhofer DH,Winkler WC
Nucleic Acids Res. 2023 Oct 13;51(18):9804-9820. doi: 10.1093/nar/gkad662. Epub
Trettel DS,Winkler WC
Microbiol Spectr. 2023 Feb 14;11(2):e0335722. doi: 10.1128/spectrum.03357-22. Epub 2023 Feb 14
Weiss CA,Myers TM,Wu CH,Jenkins C,Sondermann H,Lee VT,Winkler WC
Nucleic Acids Res. 2022 Nov 28;50(21):12369-12388. doi: 10.1093/nar/gkac1091. Epub
Lee VT,Sondermann H,Winkler WC
FEMS Microbiol Rev. 2022 Nov 2;46(6):. doi: 10.1093/femsre/fuac038. Epub
Trettel DS,Resager W,Ueberheide BM,Jenkins CC,Winkler WC
Structure. 2022 Apr 7;30(4):537-550.e5. doi: 10.1016/j.str.2022.02.002. Epub 2022 Feb 24
Lormand JD,Kim SK,Walters-Marrah GA,Brownfield BA,Fromme JC,Winkler WC,Goodson JR,Lee VT,Sondermann H
Elife. 2022 Jan 28;11:. doi: 10.7554/eLife.77092. Epub 2022 Jan 28
Kim SK,Lormand JD,Weiss CA,Eger KA,Turdiev H,Turdiev A,Winkler WC,Sondermann H,Lee VT
Elife. 2022 Jan 28;11:. doi: 10.7554/eLife.77091. Epub 2022 Jan 28
Papenfort K,Woodson SA,Schmitz RA,Winkler WC
Mol Microbiol. 2022 Jan;117(1):1-3. doi: 10.1111/mmi.14867. Epub
Lormand JD,Kim SK,Walters-Marrah GA,Brownfield BA,Fromme JC,Winkler WC,Goodson JR,Lee VT,Sondermann H
Elife. 2021 Sep 17;10:. doi: 10.7554/eLife.70146. Epub 2021 Sep 17
Elghondakly A,Wu CH,Klupt S,Goodson J,Winkler WC
J Mol Biol. 2021 Jul 23;433(15):167100. doi: 10.1016/j.jmb.2021.167100. Epub 2021 Jun 11
Weiss CA,Winkler WC
Methods Mol Biol. 2021;2323:153-170. doi: 10.1007/978-1-0716-1499-0_12. Epub
Goodson JR,Zhang C,Trettel D,Ailinger HE,Lee PE,Spirito CM,Winkler WC
Mol Microbiol. 2020 Aug;114(2):348-360. doi: 10.1111/mmi.14517. Epub 2020 May 13
Weiss CA,Hoberg JA,Liu K,Tu BP,Winkler WC
J Bacteriol. 2019 Aug 15;201(16):. doi: 10.1128/JB.00247-19. Epub 2019 Jul 24
Kim SK,Lormand JD,Weiss CA,Eger KA,Turdiev H,Turdiev A,Winkler WC,Sondermann H,Lee VT
Elife. 2019 Jun 21;8:. doi: 10.7554/eLife.46313. Epub 2019 Jun 21
Kaval KG,Gebbie M,Goodson JR,Cruz MR,Winkler WC,Garsin DA
J Bacteriol. 2019 May 15;201(10):. doi: 10.1128/JB.00703-18. Epub 2019 Apr 24