Understanding the survival, multiplication and transmission of pathogens that   are autochthonous in the environment targeted for disease prevention and intervention. Climate has significant impact on the occurrence, survival, multiplication of environmental organisms and its ability to cause disease. Studying waterborne pathogens, primary focusing on Vibrio cholerae, which is the causative agent of cholera. Conventional bacteriological, immunological and molecular methods, as well as bioinformatics and satellite remote sensing are being employed to develop a global prediction model with the ultimate goal to prevent cholera. Safe drinking water has also been a focus in my research and a simple filtration method for safe drinking water was developed, which has been proven to save lives and prevent suffering in an extensive clinical field trial carried out in Bangladesh.  

 

Huq publication (2013-2022)

 

1.     Chomvarin C, F. T. Johura, S.B. Mannan, W.  Jumroenjit, B. Kanoktippornchai, W. Tangkanakul, N. Tantisuwichwong, S.  Huttayananont, H. Watanabe, N.A. Hasan, A. Huq,  A. Cravioto, R.  Colwell,  M.  Alam.  2013. Drug response and genetic properties of Vibrio cholerae associated with endemic cholera in Northeastern Thailand, 2003-2011.  J  Med Microbiol. https://doi:10.1099/jmm.0.053801-0 

 

2.     Jutla, A., A. Akanda, A. Huq, A. G. Farouque, R. Colwell and S Islam. 2013. A water marker monitored by satellites to predict seasonal endemic cholera. J. Remote Sensing Letters, 4(8):822–831. https://doi.org/10.1080/2150704X.2013.802097

 

3.     Rowe, I, M. Elahi,  A. Huq, and S. Sukharev. 2013. The mechanoelectrical response of the cytoplasmic membrane of Vibrio cholerae. J. Gen Physiol. 142:75-85. www.jgp.org/cgi/doi/10.1085/jgp.201310985.

 

4.     Jaiani E, T. Kokashvili , N. Mitaishvili, T. Elbakidze, N. Janelidze, N.  Lashkhi,  R. Kalandadze, E. Mikashavidze, G. Natroshvili , C. A. Whitehouse, A. Huq, M. Tediashvili. 2013. Microbial water quality of recreational lakes near Tbilisi, Georgia.  J Water Health, 11(2):333-345. https://doi:10.2166/wh.2013.057  

 

5.     Akanda, Ali, A.  Jutla, D. Gute, R. Sack, M. Alam, A. Huq, R. Colwell, and S. Islam. 2013. population Vulnerability to Biannual Cholera Outbreaks and Associated Macro-Scale Drivers in Bengal Delta. Amer. J. Trop. Med Hyg, pp 89(5):950-959. https://doi:10.4269/ajtmh.12-0492

 

6.     Jutla, A., E. Whitcombe, N. Hasan, B. Haley, A. Akanda, A. Huq , M. Alam, B. Sack, R. Colwell. 2013. Environmental Factors Influencing Epidemic Cholera. Amer. J. Trop. Med. & Hyg. 89(3):597-607.  https://DOI:10.4269/ajtmh.12-0721 .

 

7.     Jutla, A.S., Whitcombe, E, Hasan, H., Haley, B., Akanda, A., Huq, A., Alam, M., Sack, B., Colwell, R. 2013. In Response: Environmental Factors Influencing Epidemic Cholera. American Journal of Tropical Medicine and Hygiene 89(6): 1231–1232. https://doi:10.4269/ajtmh.12-0721       

 

8.     Alam, M., S. M Rashed, S. B. Mannan, T. Islam, M. L. Lizarraga-Partida, G. Delgado, R. Morales., J. L. Mendez, A. Navarro, H. Watanabe, M. Ohnishi,  N. A. Hasan. A. Huq, G. B. Nair, R. B. Sack, R. R. Colwell, and A. Cravioto. 2013.  Vibrio cholerae CTX+ El Tor with an additional Truncated CTX Prophage Displaces Classical and Altered El Tor in Mexico, 1998 – 2008. J. Emerging Infect Dis.111(27):9917-22. https://doi:10.1073/pnas.1323408111    

 

9.     Hasan, A. N., D. Ceccarelli, C. J. Grim, E. Taviani, J. Choi, A.  Sadique, M. Alam, A. K. Siddique, R. B. Sack, A. Huq, and R. R. Colwell. 2013. Distribution of virulence genes in clinical and environmental Vibrio cholerae in Bangladesh. App Environ. Microbiol. 79(18):5782. https://doi:10.1128/AEM.01113-13   

 

10.  Rashed, S. M., A. Iqbal, S B. Mannan, T. Islam, M. Rashid, F. Johura, H. Watanabe, N. A. Hasan, A. Huq, O. C. Stine, R. B. Sack, R. R. Colwell, and M. Alam. 2013. Vibrio cholerae O1 El Tor and O139 Bengal Strains Carrying ctxBET, Bangladesh. J. Emerging Infect. Dis, 19(10):1713-1715.    https://DOI:10.3201/eid1910.130626  

 

11.  Ceccarelli, D., M. Spagnoletti, N. A. Hasan, S. Lansing, A. Huq, and R. R. Colwell. 2013. A new Integrative Conjugative Element Detected in Haitian Isolates of Vibrio cholerae non-O1/non-O139. Res Microbiol.  164(9):891-893. PMID: 23994142 PMCID: PMC3999591 https://DOI:10.1016/j.resmic.2013.08.004      

 

12.  Haley, B. J., S. Y. Choi, N. A. Hasan, A. S. H. Abdullah, T. A. Cebula, A. Huq, R. R. Colwell. 2013. Genome Sequences of Clinical Vibrio cholerae Isolates from an Oyster-borne Cholera Outbreak, Florida, USA. Appl. Environ. Microbiol. https://doi:10.1128/genomeA.00966-13

 

13.  Ceccarelli, D., N. A. Hasan, A. Huq, R. R. Colwell. 2013. Virulence factors of epidemic and pandemic Vibrio parahaemolyticus. Frontiers in Cellular and Infect Microbiol. 3: 97. https://doi:10.3389/fcimb.2013.00097   

 

14.  Chomvarin, C.,   W.  Jumroenjit,  W.  Namwat,  W.  Tangkanakul,  N. A. Hasan,   K.  Chaicumpar,  K. Faksri, and  A. Huq. 2014. Genotype and Drug Resistance of Clinical and Environmental Vibrio cholerae non-O1 and non-O1/O139 in Northern Thailand. The Southeast Asian J Trop Med and Pub Hlth. 45(6):1-11. PMID: 26466421

 

15.  Rashed, S. M., A. S. Azman, M. Alam, S. Li, D. A. Sack, J. G. Morris, Jr., I. Longini, A. K. Siddique, A. Iqbal, A. Huq, R. R. Colwell, R. B. Sack, O. C. Stine. 2014. Genetic Analysis of Vibrio cholerae in the Human Population of Two Rural Communities in Bangladesh During Outbreaks. J. Emerging Infect. Dis. https://doi.org/10.3201/eid2001.130796  

 

16.  Haley,  B. J., T. Kokashvili, A. Tskshvediani, N.  Janelidze, N. Mitaishvili, C. J. Grim, G. C.n de Magny, A. J. Chen, E. Taviani, T. Eliashvili, M. Tediashvili, C. Whitehouse, R. R Colwell, and A. Huq. 2014. Molecular Diversity and Predictability of Vibrio parahaemolyticus along the Georgian Coastal Zone of the Black Sea. Front. Microbiol. 10 February 2014 | https://doi:10.3389/fmicb.2014.00045     PMID: 24575085

 

17.  Hasan, N. A., T. Rezayat, p. Blatz, S. Y. Choi, K. J. Griffitt, S. M. Rashed, A. Huq, , N. Conger, R. R. Colwell, and D. J. Grimes. 2014. Non-toxigenic Vibrio cholerae non-O1/O139 Isolated from a Gulf Coast Case of Human Gastroenteritis. J. Clin. Microbiol. October, 2014. https://doi10.1128/JCM.02187-14   

 

18.  Urquhart, E. A., B. F. Zaitchika, S. D. Guikemab, B. J. Haley, E. Taviani, A. Chen, M. E. Brown, A. Huq, R. R. Colwell. 2015.  Use of Environmental Parameters to Model Pathogenic Vibrios in Chesapeake Bay. J Environmental Informatics. 26(1):1-13. https://doi:10.3808/jei.201500307     

 

19.  Kahler, A.M., B. J. Haley, A. Chen, B. J. Mull, C. L. Tarr, M. Turnsek, L. Katz, M. S. Humphrys, N. Freeman, J. Boncy, R. R. Colwell, A. Huq, and V. R. Hill. 2015. Environmental Surveillance for Toxigenic Vibrio cholerae in Surface Waters of Haiti. Amer. J. Trop. Med Hyeg. 92(1): 118-125. https://doi:10.4269/ajtmh.13-0601

 

20.  Jutla, A., A. Huq and R. R. Colwell. 2015. Diagnostic approach for monitoring hydroclimatic conditions related to emergence of West Nile virus in West Virginia. Front. Pub. Hlth. 3:10. https://doi:10.3389/fpubh.2015.00010   

 

21.  Ceccarelli, D., A. Chen, N. Hasan, S. Rashed, A. Huq, and R. Colwell. 2015. Non-O1/non-O139 Vibrio cholerae carrying multiple virulence factors and V. cholerae O1 in the Chesapeake Bay, Maryland. Appl. Environ. Microbiol. 81(6):1909-18. https://doi:10.1128/AEM.03540-14

 

22.  Hasan, N. A., C. J. Grim , E. K Lipp , I. N.G. Rivera , J. Chun, B. J. Haley , E. Taviani , S. Y. Choi , M. l Hoq , C. Munk , T. S. Brettin , D. Bruce , J. F. Challacombe , C. Detter ,C. S. Han, J. A Eisen , A. Huq , R. R. Colwell. 2015. Deep-sea Hydrothermal Vent Bacteria Related to Human Pathogenic Vibrio Species. Proc. Nat. Acad. Sc. May 112(21):E2813-9. PMID: 25964331                https://doi:10.1073/pnas.1503928112       

 

23.  Nasr-Azadani, Fariborz, A. Unnikrishnan, A. Akanda, S. Islam, M. Alam, A. Huq, A. Jutla, R. Colwell. 2015. Downscaling River discharge to assess the effects of climate change on endemic cholera. J. Climate Res. DOI: https://doi.org/10.3354/cr01310

 

24.  Assadi, M., P. Chamanrokh, C. A. Whitehouse, A. Huq. 2015. Methods for Detecting the Environmental Coccoid Form of Helicobacter pylori. Front Public Health. May 28(3):147. https://doi.org/10.3389/fpubh.2015.00147

 

25.  Jutla, Antarpreet, H. Aldach, H. Billian, R. Posa, A. Akanda, A. Huq, R. Colwell. 2015. Satellite based assessment of hydroclimatic conditions related to cholera in Zimbabwe. PLoS One. https://doi.org/10.1371/journal.pone.0137828

 

26.  Kokashvili, T., C. A. Whitehouse, A.Tskhvediani, C. J. Grim, T. Elbakidze, N. Mitaishvili, N. Janelidze, E. Jaiani, B. J. Haley, N. Lashkhi, A. Huq, R. R. Colwell and M. Tediashvili. 2015. Occurrence and diversity of clinically important Vibrio species in the aquatic environment of Georgia. Front. J. Pub. Hlth. https://doi.org/10.3389/fpubh.2015.00232

 

27.  Jutla, A., A. Ali, A. Unnikrishnan,W. Palaash, A. Huq, R. Colwell. 2015. Predictive Time Series Analysis Linking Bengal Cholera with Terrestrial Water Storage Measured from Gravity Recovery and Climate Experiment Sensors. Amer J Trop Med Hyg, 93(6):(#J. Nature Geoscience. https://doi.org/10.4269/ajtmh.14-0648

 

28.  Choi, S. Young, S. M. Rashed, N. A. Hasan, M. Alam, T. Islam, A. Sadique, F. Johura, M. Eppinger, J. Ravel, A. Huq, A. Cravioto, R. R. Colwell. 2016. Phylogenetic Diversity of Vibrio cholerae Associated with Endemic Cholera in Mexico from 1991 to 2008. mBio 7(2):e02160-15. https://doi.org/10.1128/mBio.02160-15. 

 

29.  Fariborz Nasr-Azadani, R. Khan, J. Rahimikollu, A. Unnikrishnan, J. Rahimikollu, A. Akanda, M. Alam, A. Huq, A. Jutla, R. Colwell. 2016. Hydroclimatic sustainability assessment of changing climate on cholera in the Ganges-Brahmaputra Basin. Advances in Water Resources. Advances in Water Resources 108 (2017) 332-344. https://doi.org/10.1016/j.advwatres.2016.11.018

 

30.  Rashid, M., M.  Almeida, A. Azman,; B.  Lindsay, D. Sack, R. Colwell, A. Huq, M. Alam, J. Morris, and C. Stine. 2016. Comparison of Inferred Relatedness Based on Multilocus Variable-number Tandem-repeat Analysis (MLVA) and Whole Genome Sequencing (WGS) of Vibrio cholerae. FEMS Microbiol Lett., 363 (12):116 https://doi.org/10.1093/femsle/fnw116  

 

31.  Ceccarelli, D., M. Alam, A. Huq, R. R. Colwell. 2016. Reduced susceptibility to extended-spectrum β-lactams in V. cholerae isolated in Bangladesh. Front Pub. Health. https://doi.org/10.3389/fpubh.2016.00231.

 

32.  Rashed, S. M., N. A. Hasan, M. Alam, A. Sadique, M. Sultana. M. M. Hoq, R. B. Sack, R. R. Colwell and A. Huq. 2017. Vibrio cholerae O1 with Reduced Susceptibility to Ciprofloxacin and Azithromycin Isolated from a Rural Coastal Area of Bangladesh. Front Microbiol. Feb 21, 2017. https://doi.org/10.3389/fmicb.2017.00252.

 

33.  Briquairea, R., R. R. Colwell, J. Boncy, E. Rossignold, A. Dardye, I. Pandinif, F. Villevalg, J. Machurona, A. Huq, S. Rashed. 2017. Application of a paper-based device containing a new culture medium to detect Vibrio cholerae in water samples collected in Haiti. J. Microbiol Meth.133:23–31. https://doi.org/10.1016/j.mimet.2016.12.014.

 

34.  Khan, R., R. Anwar, S. A. Akanda, M. D. McDonald, A. Huq, A. Jutla, R. R. Colwell. 2017. Assessment of Risk of Cholera in Haiti following Hurricane Matthew. The Amer J Trop Med and Hyg, 97(3) 896 – 903. DOI: https://doi.org/10.4269/ajtmh.17-0048.

 

35.  Ceccarelli, D., G. Garriss, S. Y. Choi, N. A. Hasan, R. Stepanauskas, M. Pop, A. Huq and R. R. Colwell. 2017.  Characterization of two cryptic plasmids isolated in Haiti from clinical Vibrio cholerae non-O1/non-O139. Front. Microbiol., 23 November 2017 https://doi.org/10.3389/fmicb.2017.02283. 

 

36.  Chen, A., N. A. Hasan, B. J. Haley, E. Taviani, S. Young Choi, R. McKay, M. Tarnowski, K. Brohawn, C. N. Johnson, R. R. Colwell and A. Huq. 2017. Characterization of Pathogenic Vibrio parahaemolyticus from the Chesapeake Bay, Maryland. Front. Microbiol, 15 December 2017.  https://doi.org/10.3389/fmicb.2017.02460

 

37.  Akanda, A. S., S. Aziz, A. Jutla, A. Huq, M. Alam, G. U. Ahsan, and R. Colwell. 2018. Satellites and cell phones form a cholera early-warning system, Eos, 99,  https://doi.org/10.1029/2018EO094839.

 

38.  Hossain, M., M. Alam, A. Khaleque, S. Islam, A. Sadique, N. Khana, Z. Halima, M. Sarker, N. M. El-Sayed, A. Huq, G. U. Ahsan, and R. R. Colwell. 2018. Virulence-Related Genes Identified from the Genome Sequence of the Non-O1/Non-O139 Vibrio cholerae Strain VcN1, Isolated from Dhaka, Bangladesh. Genome Announc. March 2018 vol. 6 no. 10 e01513-17. https://doi.org/10.1128/genomeA.01513-17. 

 

39.  Sultana, M., S. Nusrin, N. A. Hasan, A. Sadique, K. U. Ahmed, A. Islam,  A. Hossain, I. Longini, A. Nizam, A. Huq, A. K. Siddique, D. A. Sack, R. B. Sack,  R. R. Colwell, and M. Alam. 2018. Biofilms Comprise a Component of the Annual Cycle of Vibrio cholerae in the Bay of Bengal Estuary. March/April, mBio, 9(2):1-13. https://doi.org/10.1128/mBio.00483-18.

 

40.  Chen, A., M. Tamburri, R. R. Colwell and A. Huq. 2018. Potential application of SMART II for Vibrio cholerae O1 and O139 detection in ship's ballast water. Mar. Poll. November Bull. 136:79-83. https://doi.org/10.1016/j.marpolbul.2018.08.029.

 

41.  Khan R, Aldaach H, McDonald C, Alam M, Huq A, Gao Y, Akanda AS, Colwell R, Jutla A. 2019. Estimating cholera risk from an exploratory analysis of its association with satellite-derived land surface temperatures International Journal of Remote Sensing. 40: 4898-4909.     https://doi.org/10.1080/01431161.2019.1577575 

 

42.  Brumfield, K. D., A. Huq, R. R. Colwell, J. L. Olds, M. B. Leddy. 2020. Microbial resolution of whole genome shotgun and 16S amplicon metagenomic sequencing using publicly available NEON data. PLoS ONE, 15(2): e0228899 https://doi.org/10.1371/journal.pone.0228899

 

43.  Brumfield, K. D, N. A, Hasan, M. B, Leddy, J. A, Cotruvo, S. M, Rashed,  R.R, Colwell,  and A. Huq. 2020. A comparative analysis of drinking water employing metagenomics. PLoS ONE 15(4): e0231210. https://doi.org/10.1371/journal.pone.0231210

 

44.  Brumfield, K. D., J. A. Cotruvo, M. B. Leddy, R. R. Colwell, and A. Huq. 2020. Using Metagenomic Analysis to Assess Water Quality. J. Amer Water Works Assoc (AAWA), 112:11, 84-87. https://doi.org/10.1002/awwa.1618

 

45.  M, Tabassum T, Rahman A, Hossain A, Afroze T, Momen AMI, Sadique A, Sarker M, Shams F, Ishtiaque A, Khaleque A, Alam M, Huq A, Ahsan GU, Colwell RR. 2020. Genotype-phenotype correlation of β-lactamase-producing uropathogenic Escherichia coli (UPEC) strains from Bangladesh. Scientific Reports. 10: 14549. PMID 32883963   https://doi.org/10.1038/s41598-020-71213-5                     

 

46.  Aziz S, Pakhtigian EL, Akanda AS, Jutla A, Huq A, Alam M, Ashan GU, Colwell RR. 2021. Does improved risk information increase the value of cholera prevention? An analysis of stated vaccine demand in slum areas of urban Bangladesh. Social Science & Medicine (1982). 272: 113716. PMID 33571944   https://doi.org/10.1016/j.socscimed.2021.113716    

 

47.  Al Malki A, Brumfield KD, Tsui CKM, Anand A, Rashed SM, Ibrahim E, Al Shamari H, Huq A, Colwell RR, Fotedar R. 2021. Draft Genome Sequences of Seven Vibrio cholerae Isolates from Adult Patients in Qatar. Microbiology Resource Announcements. 10. PMID 33664147 https://doi.org/10.1128/MRA.01489-20  

 

48.  Brumfield, K. D, J. A, Cotruvo, O. C. Shanks, M. Sivangesan, J. Hey, N. A, Hasan, A. Huq R.R, Colwell, and M. Leddy. 2021. Metagenomic Sequencing and Quantitative Real-Time PCR for Fecal Pollution Assessment in an Urban Watershed. February 2021. Frontiers in Water 3:626849. https://doi.org/10.3389/frwa.2021.626849

 

49.  Sadique A, Neogi SB, Bashar T, Sultana M, Johura FT, Islam S, Hasan NA, Huq A, Colwell RR, Alam M. 2021 Dynamics, Diversity, and Virulence of in Homestead Pond Water in Coastal Bangladesh. Frontiers in Public Health. 9: 692166. PMID 34307285  https://doi.org/10.3389/fpubh.2021.692166

 

51.   Brumfield KD, Usmani M, Chen KM, Gangwar M, Jutla AS, Huq A, Colwell RR. 2021. Environmental parameters associated with incidence and transmission of pathogenic Vibrio spp. Environmental Microbiology. 23:12, 7314-7340. PMID 34390611 https://doi.org/10.1111/1462-2920.15716

 

52.   Usmani M, Brumfield KD, Jamal Y, Huq A, Colwell RR, Jutla A. 2021. A Review of the Environmental Trigger and Transmission Components for Prediction of Cholera. Tropical Medicine and Infectious Disease. 6(3), p.147. https://doi.org/10.3390/tropicalmed6030147

 

53.   Chamanrokh, P., R. R. Colwell and A. Huq. 2021. Loop Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of VBNC cells of Vibrio cholerae O1 in Water. Can J. Microbiol. • 18 November 2021 • https://doi.org/10.1139/cjm-2021-0142

 

54.   Mamun, M. M., T. Hossain, M. Morita, M. Ohnishi, F.Tuz Johura, M. Sultana, S. Monira, T. Ahmed, N. Thomson, H. Watanabe, A. Huq, R. R. Colwell.  2022. Genomic Characteristics of Recently Recognized Vibrio cholerae El Tor Lineages Associated with Cholera in Bangladesh, 1991 to 2017. Microbiol Spectr. 10(2):e0039122. Apr 2022. PMID: 35315699 PMCID: PMC9045249 DOI: 10.1128/spectrum.00391-22

 

55.   Usmani, M., K. D. Brumfield, ,B. M. Magers, A. Huq, R. Barciela, T. H. Nguyen, R. R. Colwell and A. Jutla. 2022. Predictive Intelligence for Cholera in Ukraine? GeoHealth, September 2022. https://doi.org/10.1029/2022GH000681

 

56.   Johura, Fatema-Tuz, S. R. Biswas, S. M. Rashed. M. T. Islam, S. Islam. M. Sultana, H Watanabe, A, Huq, N. R. Thomson, R. R.Colwell, and M. Alam. 2022. Vibrio cholerae O1 El Tor strains linked to global cholera show region-specific patterns by pulsed-field gelelectrophoresis. Infection, Genetics and Evolution, Vol.105, November 2022.https://doi.org/10.1016/j.meegid.2022.105363

 

57.   Brumfield, K. D, M. Leddy, M. Usmani, J. A Cotruvo, C.-Tzone Tien, S. Dorsey, K. Graubics, B. Fanelli, I. Zhou, N. Registe, M. Dadlani, M. Wimalarante, D. Jinasena, R. Abayagunawardena, C..Withanachchi, A. Huq, A. Jutla, R. R Colwell. 2022.  Microbiome analysis for wastewater surveillance during COVID-19. MBio 13 (4), e00591-22 DOI: https://doi.org/10.1128/mbio.00591-22

 

58.   Alam M., M. M. Monir, M. T. Islam, R. Mazumder, D. Mondal, K. S. Nahar, M. Sultana, M. Morita, M. Ohnishi, A. Huq, H. Watanabe, F. Qadri, M. Rahman, N. Thomson, K. Seed, R. R Colwell, T. Ahmed. 2022. Genomic attributes of Vibrio cholerae responsible for a massive cholera outbreak in Bangladesh, Sept 2022.  DOI: https://doi.org/10.21203/rs.3.rs-1878982/v1

 

59.   Brumfield, K.D., Raupp, M.J., Haji, D., Simon, C., Graf, J., Cooley, J.R., Janton, S.T., Meister, R.C., Huq, A., Colwell, R.R. and Hasan, N.A., 2022. Gut microbiome insights from 16S rRNA analysis of 17-year periodical cicadas (Hemiptera: Magicicada spp. Broods II, VI, and X. Scientific reports, 12(1), pp.1-16. https://doi:10.1038/s41598-022-20527-7