Jeffrey DeStefano
Contact Info
Office: 3130 Bioscience Research Bldg.
Phone: 301-405-5449
DeStefano Lab
Jeffrey DeStefano
Professor

Teaching

  • General Virology (BSCI437)
  • Graduate Virology (CBMG688K)

Graduate Program Affiliations

  • BISI-Molecular & Cellular Biology (MOCB)

Research Interests

HIV and poliovirus replication and recombination

Education

  • Ph.D., University of Connecticut, 1990

All Publications

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Latest Publications

Razi N,Li W,Ignacio MA,Loube JM,Agostino EL,Zhu X,Scull MA,DeStefano JJ
Inhibition of SARS-CoV-2 infection in human airway epithelium with a xeno-nucleic acid aptamer.
Respir Res. 2023 Nov 7;24(1):272. doi: 10.1186/s12931-023-02590-4. Epub 2023 Nov 7
Razi N,Li W,Ignacio MA,Loube JM,Agostino EL,Zhu X,Scull MA,DeStefano JJ
Inhibition of SARS-CoV-2 Infection in Human Airway Epithelium with a Xeno-Nucleic Acid Aptamer.
bioRxiv. 2023 Sep 28;:. pii: 2023.09.27.559799. doi: 10.1101/2023.09.27.559799. Epub 2023 Sep 28
Harrison JJEK,Passos DO,Bruhn JF,Bauman JD,Tuberty L,DeStefano JJ,Ruiz FX,Lyumkis D,Arnold E
Cryo-EM structure of the HIV-1 Pol polyprotein provides insights into virion maturation.
Sci Adv. 2022 Jul 8;8(27):eabn9874. doi: 10.1126/sciadv.abn9874. Epub 2022 Jul 6
Ruiz FX,Hoang A,Dilmore CR,DeStefano JJ,Arnold E
Structural basis of HIV inhibition by L-nucleosides: Opportunities for drug development and repurposing.
Drug Discov Today. 2022 Jul;27(7):1832-1846. doi: 10.1016/j.drudis.2022.02.016. Epub 2022 Feb 23
Oliveira R,Pinho E,Sousa AL,DeStefano JJ,Azevedo NF,Almeida C
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches.
Trends Biotechnol. 2022 May;40(5):549-563. doi: 10.1016/j.tibtech.2021.09.011. Epub 2021 Oct 28
DeStefano JJ,Iseni F,Tarbouriech N
Selection of Primer-Template Sequences That Bind with Enhanced Affinity to Vaccinia Virus E9 DNA Polymerase.
Viruses. 2022 Feb 10;14(2):. doi: 10.3390/v14020369. Epub 2022 Feb 10
Wang R,Belew AT,Achuthan V,El Sayed N,DeStefano JJ
Physiological magnesium concentrations increase fidelity of diverse reverse transcriptases from HIV-1, HIV-2, and foamy virus, but not MuLV or AMV.
J Gen Virol. 2021 Dec;102(12):. doi: 10.1099/jgv.0.001708. Epub
Alves Ferreira-Bravo I,DeStefano JJ
Xeno-Nucleic Acid (XNA) 2'-Fluoro-Arabino Nucleic Acid (FANA) Aptamers to the Receptor-Binding Domain of SARS-CoV-2 S Protein Block ACE2 Binding.
Viruses. 2021 Oct 2;13(10):. doi: 10.3390/v13101983. Epub 2021 Oct 2
Harrison JJEK,Tuske S,Das K,Ruiz FX,Bauman JD,Boyer PL,DeStefano JJ,Hughes SH,Arnold E
Crystal Structure of a Retroviral Polyprotein: Prototype Foamy Virus Protease-Reverse Transcriptase (PR-RT).
Viruses. 2021 Jul 29;13(8):. doi: 10.3390/v13081495. Epub 2021 Jul 29
Ferreira-Bravo IA,DeStefano JJ
Xeno-nucleic Acid (XNA) 2'-Fluoro-Arabino Nucleic Acid (FANA) Aptamers to the Receptor Binding Domain of SARS-CoV-2 S Protein Block ACE2 Binding.
bioRxiv. 2021 Jul 14;:. pii: 2021.07.13.452259. doi: 10.1101/2021.07.13.452259. Epub 2021 Jul 14
Dilmore CR,DeStefano JJ
HIV Reverse Transcriptase Pre-Steady-State Kinetic Analysis of Chain Terminators and Translocation Inhibitors Reveals Interactions between Magnesium and Nucleotide 3'-OH.
ACS Omega. 2021 Jun 8;6(22):14621-14628. doi: 10.1021/acsomega.1c01742. Epub 2021 May 25
Tuske S,Zheng J,Olson ED,Ruiz FX,Pascal BD,Hoang A,Bauman JD,Das K,DeStefano JJ,Musier-Forsyth K,Griffin PR,Arnold E
Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer.
Curr Res Struct Biol. 2020;2:116-129. doi: 10.1016/j.crstbi.2020.06.002. Epub 2020 Jun 30
Rose KM,Alves Ferreira-Bravo I,Li M,Craigie R,Ditzler MA,Holliger P,DeStefano JJ
Selection of 2'-Deoxy-2'-Fluoroarabino Nucleic Acid (FANA) Aptamers That Bind HIV-1 Integrase with Picomolar Affinity.
ACS Chem Biol. 2019 Oct 18;14(10):2166-2175. doi: 10.1021/acschembio.9b00237. Epub 2019 Oct 7
DeStefano JJ
Non-nucleoside Reverse Transcriptase Inhibitors Inhibit Reverse Transcriptase through a Mutually Exclusive Interaction with Divalent Cation-dNTP Complexes.
Biochemistry. 2019 Apr 23;58(16):2176-2187. doi: 10.1021/acs.biochem.9b00028. Epub 2019 Apr 5
Das K,Martinez SE,DeStefano JJ,Arnold E
Structure of HIV-1 RT/dsRNA initiation complex prior to nucleotide incorporation.
Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7308-7313. doi: 10.1073/pnas.1814170116. Epub 2019 Mar 22
DeStefano JJ,Alves Ferreira-Bravo I
A highly sensitive aptamer-based HIV reverse transcriptase detection assay.
J Virol Methods. 2018 Jul;257:22-28. doi: 10.1016/j.jviromet.2018.04.005. Epub 2018 Apr 6
Fenstermacher KJ,Achuthan V,Schneider TD,DeStefano JJ
An Evolutionary/Biochemical Connection between Promoter- and Primer-Dependent Polymerases Revealed by Systematic Evolution of Ligands by Exponential Enrichment.
J Bacteriol. 2018 Apr 1;200(7):. doi: 10.1128/JB.00579-17. Epub 2018 Mar 12