During the last several years, we have upgraded the research facilities throughout the department and college. We have created several state-of the-art shared instrumentation laboratories that enable our students and faculty to have access to sophisticated instruments whose purchase and maintenance costs far exceed the budgets of individual investigators. Two of these shared instrument laboratories center around biological imaging, for both electron and light microscopy, including brand-new a field-emission scanner, a new confocal microscope and an image reconstruction/deconvolution microscope. A newly established shared laboratory augments existing sequencing facilities on campus and serves the molecular geneticists for the large-scale processing and sequencing of nucleic acids, with multiple robotic sequenators and real time PCR.
Other core facilities in the department and elsewhere on campus provide instrumentation for fluorescence-activated cell sorting (FACS) and analysis, NMR, and mass spectrometry. Equipment and analytical instruments are available in both faculty and core laboratories for the maintenance of animal and plant tissue cultures, for the production of monoclonal antibodies, for the synthesis and micro-analysis of proteins, for large-scale fermentation and cultivation of microorganisms, and for computer assisted molecular modeling. Support staffing in shared instrumentation facilities is provided by the college, and maintenance costs have been subsidized by the college, thereby providing even occasional users with appropriate training and access, and simultaneously, keeping instrument use costs low. We have found that this strategy provides exceptional opportunities for research and training, and enables our students to perform experiments with instrumentation that is at the leading edge of technology.
The Proteomics Core Facility is located in room 0109/0111 Biology-Psychology Building. The facility is equipped with a state-of-art ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometer that is interfaced to a Dionex Ultimate3000 RSLCnano HPLC system. The facility also maintains 2 separate data stations dedicated for proteomics data processing, database searching, and generation of reports.
The Imaging Core (IC) provides researchers in the Life Sciences with the opportunity to image live, fixed, unlabeled or fluorescently labeled samples using one of several conventional and advanced light microscope systems, including laser scanning confocal and total internal reflection.
The Flow Cytometry is located in room 2102 Bioscience Research Building. The facility is equipped with state-of-art FACSCAnto II, FACSAria II and Luminex Magpix.
The Flow Cytometry is located in room 0107 Microbiology Building. The facility is equipped with state-of-art Agilent 4150 TapeStation System, Roche LightCycler 480 Real-Time
qPCR machine, Covaris S220 Focused-ultrasonicator and BioRad CFX96 qPCR.