Vincent Lee

Associate Professor


Office Phone: (301) 405-9397

Fax: (301) 314-1248

Office Address: 3114 Biosciences Research Building



BSCI412 Microbial Genetics

Graduate Program Affiliations

  • BISI - BISI-Molecular & Cellular Biology (MOCB)


Research Interests

Host-pathogen interactions, molecular mechanisms of pathogenesis for Pseudomonas aeruginosa, allosteric regulation of molecular complexes, bacterial signal transduction and small molecular inhibitors.


Recent Publications


Roelofs, K.G., Jones, C.J., Helman, S.H., Shang, X., Orr, M.W., Goodson, J.R., Galperin, M.Y., Yildiz, F.H., and Lee, V.T. 2015. Systematic identification of cyclic-di-GMP binding proteins in Vibrio cholerae reveals a novel class of cyclic-di-GMP-binding ATPases associated with Type II secretion systems. PLoS Pathogens.


Zhou, W., Orr, M.W., Jian, G., Watt, S.K., Lee, V.T., Zachariah, M.R. 2015 Inactivation of bacterial spores subjected to sub-second thermal stress. Chemical Engineering Journal. 279(2015):578–588


Orr, M.W., Donaldson, G.P., Severin, G.B., Wang, J., Sintim, H.O., Waters, C.M., and Lee, V.T. 2015. Oligoribonuclease is the primary degradative enzyme for pGpG in Pseudomonas aeruginosa that is required for cyclic-di-GMP turnover. Proc Natl Acad Sci U S A.


Cole, S.J., Lee, V.T. 2015. Cyclic-di-GMP signaling contributes to Pseudomonas aeruginosa mediated catheter-associated urinary tract infection. J Bacteriol. JB.00410-15. Epub 2015 July 20.

Wang, H., Jian, G., Zhou, W., DeLisio, J., Lee, V.T., Zachariah, M.R. 2015. Metal Iodate-based Energetic Composites and their Combustion and Biocidal Performance. ACS Appl Mater Interfaces. Epub 2015 Jul 10.

Cole, S.J., Records, A.R., Orr, M.W., Linden, S.B., and Lee, V.T. 2014. Catheter-associated urinary tract infection by Pseudomonas aeruginosa is mediated by exopolysaccharide-independent biofilms. Infect Immun. 82(5):2048-58. doi: 10.1128/IAI.01652-14. Epub 2014 Mar 4.

Lieberman, O.J., Orr, M.W., Wang, Y., and Lee, V.T. 2014. High-throughput screening using the differential radial capillary action of ligand assay identifies ebselen as an inhibitor of diguanylate cyclases. ACS Chemical Biology. 9(1):183-92. doi: 10.1021/cb400485k. Epub 2013 Nov 4.

Corrigan, R.M., Campeotto, I., Jeganathan, T., Roelofs, K.G., Lee, V.T. and Gründling, A. 2013. Systematic identification of conserved bacterial c-di-AMP receptor proteins. Proc Natl Acad Sci U S A. 110(22):9084-9. Epub 2013 May 13.

Zhou, J., Watt, S.K., Wang, J., Nakayama, S., Sayre, D.A., Lam, Y.-F., Lee, V.T., Sintim, H.O. 2013. Potent suppression of c-di-GMP synthesis via I-site allosteric inhibition of diguanylatecyclases with 2’-F-c-di-GMP. Bioorganic & Medicinal Chemistry. 21(14):4396-404. doi: 10.1016/j.bmc.2013.04.050. Epub 2013 Apr 29.


Lieberman, O.J., DeStefano, J.J., and Lee, V.T. 2013. Detection of cyclic diguanylate G-octaplex assembly and interaction with proteins. PLoS One. 8(1):e53689. doi: 10.1371/journal.pone.0053689. Epub 2013 Jan 7.


Zhou, W., Watt, S.K., Tsai D.-H., Lee, V.T., Zachariah, M.R. 2012 Quantitative attachment and detachment of bacterial spores from fine wires through pulsed voltammetry. J Phys Chem B. 117(6):1738-45. doi: 10.1021/jp307282q. Epub 2012 Nov 9.


Nakayama S., Roelofs K., Lee V.T., Sintim H.O. 2012. A c-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity-implication for a simple colorimetric detection. Molecular BioSystems. 8(3):726-9. doi: 10.1039/c2mb05430b. Epub 2012 Jan 18.


Donaldson, G.P., Roelofs, K.G., Luo, Y., Sintim, H.O., Lee, V.T. 2011. A Rapid Assay for Affinity and Kinetics of Molecular Interactions with Nucleic Acids. Nucleic Acids Res. PMID: 22210888


Luo, Y., Zhang, J., Watt, S.K., Lee V.T., Sintim H.O. 2011. Differential binding of 2’-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins. Moleular BioSystems. PMID: 22182995


Roelofs, K.G., Wang, J., Sintim, H.O., Lee, V.T. 2011. Differential radial capillary action of ligand assay for high-throughput detection of metabolite interactomes. Proc Natl Acad Sci U S A. 108(37):15528-33. PMID: 21876132 


Wang, J., Zhou, J., Donaldson, G., Nakayama, S., Yan, L., Lam, Y-F., Lee V.T., Sintim, H.O. 2011. Conservative Change to the Phosphate Moiety of c-di-GMP Remarkably Affects Its Polymorphism and Binding Abilities to DGC, PDE and PilZ Proteins. J Am Chem Soc.133(24):9320-30. PMID: 21612220


Nakayama S, Kelsey I, Wang J, Roelofs K, Stefane B, Luo Y, Lee V.T., Sintim HO. 2011. Thiazole orange- induced c-di-GMP quadruplex formation facilitates a simple fluorescent detection of this ubiquitous biofilm regulating molecule. J Am Chem Soc. 133(13): 4856-64. PMID: 21384923


Lee V.T. and Kessler J.L. 2009. Type III secretion systems as targets for novel therapeutics. IDrugs. 12(10): 636-41. PMID: 19790014


Salzberg, S., Sommer, D.D., Puiu, D., and Lee, V.T. 2008. Gene-boosted assembly of a novel bacterial genome from very short reads. PLoS Computational Biology. 4:e1000186. PMCID: PMC2529408 


Lee V.T. and Kessler J.L. 2009. Type III secretion systems as targets for novel therapeutics. IDrugs. 12(10): 636-41. PMID: 19790014


Merighi, M., Lee, V.T., Hyodo, M., Hayakawa, Y., and Lory, S. 2007. The second messenger bis-(3’-5’)-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa. Mol. Microbiol. 65: 876–895.


Lee, V.T., Matewish, J.M., Kessler, J.L. Hyodo, M., Hayakawa, Y., and Lory, S. 2007. A Cyclic-di-GMP Receptor Required for Bacterial Exopolysaccharide Production. Mol. Microbiol. 65: 1474-1484.


Arnoldo, A., Curak, J., Kittanakom, S., Chevelev, I., Lee, V.T., Sahebol-Amri, M., Koscik, B., Ljuma, L., Roy, P.J., Bedalov, A., Nislow, C., Merrill, R.A., Lory, S. and Stagljar, I. 2007.  Identification of small molecule inhibitors of Pseudomonas aeruginosa Exotoxin S using a yeast phenotypic screen. PLoS Genetics. 2:e1000005.



Ph.D. - University of California - Los Angeles, 2000