College of Computer, Mathematical, and Natural Sciences

Najib M. El-Sayed, Ph.D.

Associate Professor

Contact

Office Phone: (301) 405-2999
Fax: (301) 405-0745
Office Address: 3128 Biosciences Research Building

Teaching

CBMG688Y Bioinformatics and Genomics (Graduate)

CBMG688P Programming for Biology (Graduate)

BSCI380 Bioinformatics and Integrated Genomics

BSCI348R Microbiology Internship

Graduate Program Affiliations

  • BISI - BISI-Computational Biology, Bioinformatics, & Genomics (CBBG)
  • BISI - BISI-Molecular & Cellular Biology (MOCB)

Research Interests

My research program is focused on the study of the biology of parasitism and host-pathogen interactions using genomic approaches with the ultimate goal of better understanding infection and survival mechanisms. These approaches include the development and application of molecular, computational and phylogenetic tools. In the long term, our research will contribute to better diagnosis, prevention and therapeutics of parasite- and bacteria-caused diseases in humans, animals and plants.

Recent Publications

Bartholomeu, D.C., Cerqueira, G.C., Leao, A.C.A., daRocha, W.D., Pais, F.S., Macedo, C., Djikeng, A., Teixeira, S.M.R., El-Sayed, N.M. (2009) Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi. Nucleic Acids Res. 37:3407-17.

Waisberg, M., Lobo, F.P., Cerqueira, G.C., Passos, L.K., Carvalho, O.S., El-Sayed, N.M., Franco, G.R. (2008) Schistosoma mansoni: Microarray analysis of gene expression induced by host sex. Exp. Parasitol. 120:357-63.

Bringaud, F., Ghedin, E., El-Sayed, N.M., Papadopoulou, B. (2008) Role of transposable elements in trypanosomatids. Microbes Infect. 10:575-81.

Cerqueira, G.C., Bartholomeu, D.C., Darocha, W.D., Hou, L., Freitas-Silva, D.M., Machado, C.R., El-Sayed, N.M., Teixeira, S.M. (2008) Sequence diversity and evolution of multigene families in Trypanosoma cruzi. Mol. Biochem. Parasitol. 157:65-72.

Bringaud, F., Müller, M., Cerqueira, G.C., Smith, M., Rochette, A., El-Sayed, N.M., Papadopoulou, B., Ghedin, E. (2007). Members of a large retroposon family are determinants of post-transcriptional gene expression in Leishmania. (2007). PLoS Pathog. 3:1291-307.

Ghedin, E., Wang, S., Spiro, D., Caler, E., Zhao, Q., Crabtree, J., Allen, J.E., Delcher, A.L., Guiliano, D.B., Miranda-Saavedra, D., Angiuoli, S.V., Creasy, T., Amedeo, P., Haas, B., El-Sayed, N.M., Wortman, J.R., Feldblyum, T., Tallon, L., Schatz, M., Shumway, M., Koo, H., Salzberg, S.L., …, Williams, S.A., Fraser-Liggett, C., Slatko, B., Blaxter, M.L., Scott, A.L.(2007). Draft genome of the filarial nematode parasite Brugia malayi. Science 317:1756-60.

Haas, B.J., Berriman, M., Hirai, H., Cerqueira, G.C, Loverde, P.T., El-Sayed, N.M. (2007). Schistosoma mansoni genome: Closing in on a final gene set. Exp Parasitol. 117:225-8.

Waisberg, M., Lobo, F.P., Cerqueira, G.C., Passos, L.K., Carvalho, O.S., Franco, G.R., El-Sayed, N.M. (2007). Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni. BMC Genomics. 8:181.

Souza, R.T., Santos, M.R.M., Lima, F.M., El-Sayed, N.M., Myler, P.J., Ruiz, J.C., da Silveira, J.F. (2007) New Trypanosoma cruzi repeated element that shows site specificity for insertion. Euk. Cell 6:1228-38.

Stelter, K., El-Sayed, N.M., Seeber, F. (2007). The expression of a plant-type ferredoxin redox system provides molecular evidence for a plastid in the early dinoflagellate Perkinsus marinus. Protist 58:119-130.

Djikeng, A., Raverdy, S., Foster, J., Bartholomeu, D., Zhang, Y., El-Sayed, N.M., Carlow, C. (2007). Cofactor-independent Phosphoglycerate Mutase is an essential gene in procyclic form Trypanosoma brucei. Parasitol. Res. 100:887-892.

Attardo, G.M., Strickler-Dinglasan, P., Perkin, S.A., Caler, E., Bonaldo, M.F., Soares, M.B., El-Sayed, N.M., Aksoy, S. (2006). Analysis of fat body transcriptome from the adult tsetse fly, Glossina morsitans morsitans. Insect Mol. Bio. 15:411-424.

Westenberger, S.J., Cerqueira, G.C., El-Sayed, N.M., Zingales, B., Campbell, D.A., Sturm, N.R. (2006). Trypanosoma cruzi mitochondrial maxicircles display species- and strain-specific variation and possess a conserved element in the non-coding region. BMC Genomics 7:60.

Baida, R.C., Santos, M.R., Carmo, M.S., Yoshida, N., Ferreira, D., Ferreira, A.T., El-Sayed, N.M., Andersson, B., da Silveira, J.F. (2006). Molecular characterization of serine-, alanine-, and proline-rich proteins of Trypanosoma cruzi and their possible role in host cell infection. Infect. Immun. 74:1537-46.

Wu, W., Niles, E.G., El-Sayed, N., Berriman, M., LoVerde, P. (2006). Schistosoma mansoni nuclear receptors: Sixteen new members and a novel subfamily. Gene 366:303-315.

Bringaud, F., Ghedin, E., Blandin, G., Bartholomeu, D., Levin, M.J., Baltz, T., El-Sayed, N.M. (2005). Evolution of non-LTR retrotransposons in the trypanosomatid genomes:Leishmania major lost the active elements. Mol. Biochem. Parasitol. 145:158-70. [Epub 2005 Oct 10]

Bringaud, F., Bartholomeu, D., Blandin, G., Delcher, A., Baltz, T., El-Sayed, N.M., Ghedin, E. (2006). The Trypanosoma cruzi L1Tc and NARTc non-LTR retrotransposons show relative site specificity for insertion. Mol. Biol. Evol. 23:411-420. [Epub 2005 Nov 2]

MacLeod, A., Tweedie, A., McLellan, S., Taylor, S., Hall, N., Berriman, M., El-Sayed, N.M., Hope, M., Turner, C.M.R., Tait, A. (2005). The genetic map and comparative analysis with physical map of Trypanosoma brucei. Nuc. Acids. Res. 33:6688-93. [Epub 2005 Nov 27]

Barry, J.D., Marcello, L., Morrison, L.J., Read, A.F., Lythgoe, K., Jones, N., Carrington, M., Blandin, G., Bohme, U., Caler, E., Hertz-Fowler, C., Renauld, H., El-Sayed, N., Berriman, M. (2005). What the genome sequence is revealing about trypanosome antigenic variation. Biochem. Soc. Trans. 33:986-9.

El-Sayed N.M., Myler, P.J., Blandin, G., Berriman, M., Crabtree, J., Aggarwal, G., Caler, E., Renauld, H., Worthey, E.A., Hertz-Fowler, C., Ghedin, E., Peacock, C., Bartholomeu, D.C., Haas, B.J., Tran, A.N., Wortman, J.R., Alsmark, U.C., Angiuoli, S., Anupama, A., Badger, J., Bringaud, F., Cadag, E., Carlton, J.M., Cerqueira, G.C., Creasy, T., Delcher, A.L., Djikeng, A., Embley, T.M., Hauser, C., Ivens, A.C., Kummerfeld, S.K., Pereira-Leal, J.B., Nilsson, D., Peterson, J., Salzberg, S.L., Shallom, J., Silva, J.C., Sundaram, J., Westenberger, S., White, O., Melville, S.E., Donelson, J.E., Andersson, B., Stuart, K.D., Hall, N. (2005). Comparative genomics of trypanosomatid parasitic protozoa. Science 309:404-409.

El-Sayed N.M., Myler, P.J., Bartholomeu, D.C., Nilsson, D., Aggarwal, G., Tran, A.N., Ghedin, E., Worthey, E.A., Delcher, A.L., Blandin, G., Westenberger, S.J., Caler, E., Cerqueira, G.C., Branche, C., Haas, B., Anupama, A., Arner, E., Aslund, L., Attipoe, P., Bontempi, E., Bringaud, F., Burton, P., Cadag, E., Campbell, D.A., Carrington, M., Crabtree, J., Darban, H., da Silveira, J.F., De Jong, P., Edwards, K., Englund, P.T., Fazelina, G., Feldblyum, T., Ferella, M., Frasch, A.C., Gull, K., Horn, D., Hou, L., Huang, Y., Kindlund, E., Klingbeil, M., Kluge, S., Koo, H., Lacerda, D., Levin, M.J., Lorenzi, H., Louie, T., Machado, C.R., McCulloch, R., McKenna, A., Mizuno, Y., Mottram, J.C., Nelson, S., Ochaya, S., Osoegawa, K., Pai, G., Parsons, M., Pentony, M., Pettersson, U., Pop, M., Ramirez, J.L., Rinta, J., Robertson, L., Salzberg, S.L., Sanchez, D.O., Seyler, A., Sharma, R., Shetty, J., Simpson, A.J., Sisk, E., Tammi, M.T., Tarleton, R., Teixeira, S., Van Aken, S., Vogt, C., Ward, P.N., Wickstead, B., Wortman, J., White, O., Fraser, C.M., Stuart, K.D., Andersson, B. (2005). The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science 309:409-415.

Berriman M., Ghedin, E., Hertz-Fowler, C., Blandin, G., Renauld, H., Bartholomeu, D.C., Lennard, N.J., Caler, E., Hamlin, N.E., Haas, B., Bohme, U., Hannick, L., Aslett, M,A., Shallom, J., Marcello, L., Hou, L., Wickstead, B., Alsmark, U.C., Arrowsmith, C., Atkin, R.J., Barron, A.J., Bringaud, F., Brooks, K., Carrington, M., Cherevach, I., Chillingworth, T.J., Churcher, C., Clark, L.N., Corton, C.H., Cronin, A., Davies, R.M., Doggett, J., Djikeng, A., Feldblyum, T., Field, M.C., Fraser, A., Goodhead, I., Hance, Z., Harper, D., Harris, B.R., Hauser, H., Hostetler, J., Ivens, A., Jagels, K., Johnson, D., Johnson, J., Jones, K., Kerhornou, A.X., Koo, H., Larke, N., Landfear, S., Larkin, C., Leech, V., Line, A., Lord, A., Macleod, A., Mooney, P.J., Moule, S., Martin, D.M., Morgan, G.W., Mungall, K., Norbertczak, H., Ormond, D., Pai, G., Peacock, C.S., Peterson, J., Quail, M.A., Rabbinowitsch, E., Rajandream, M.A., Reitter, C., Salzberg, S.L., Sanders, M., Schobel, S., Sharp, S., Simmonds, M., Simpson, A.J., Tallon, L., Turner, C.M., Tait, A., Tivey, A.R., Van Aken, S., Walker, D., Wanless, D., Wang, S., White, B., White, O., Whitehead, S., Woodward, J., Wortman. J., Adams, M.D., Embley, T.M., Gull, K., Ullu, E., Barry, J.D., Fairlamb, A.H., Opperdoes, F., Barrell, B.G., Donelson, J.E., Hall, N., Fraser, C.M., Melville, S.E., El-Sayed N.M. (2005). The genome of the African trypanosome Trypanosoma brucei. Science 309:416-22.

Boonyaratanakornkit, B.B., Simpson, A.J., Whitehead, T.A., Fraser, C.M., El-Sayed, N.M. and Clark, D.S. 2005. Transcriptional profiling of the hyperthermophilic methanarchaeon Methanococcus jannaschii in response to lethal heat and non-lethal cold shock. Environ. Microbiol.7:789-797.

Ouhammouch, M., Langham, G.E.,Hausner, W., Simpson, A.J., El-Sayed, N.M. & Geidushek, P. 2005. Promoter architecture and response to a positive regulator of archaeal transcription. Mol. Microbiol. 56:625-637.

Loftus, B., Anderson, I., Davies, R., Alsmark, U.C., Samuelson, J., Amedeo, P., Roncaglia, P., Berriman, M., Hirt, R.P., Mann, B.J., Nozaki, T., Suh, B., Pop, M., Duchene, M., …[25 authors]…, Stroup, S.E., Bhattacharya, S., Lohia, A., Foster, P.G., Sicheritz-Ponten, T., Weber, C., Singh, U., Mukherjee, C., El-Sayed, N.M., Petri, W.A. Jr, Clark, C.G., Embley, T.M., Barrell, B., Fraser, C.M., Hall, N. 2005. The genome of the protist parasite Entamoeba histolytica. Nature 433:865-868.

Kim, D., Chiurillo, M.A., El-Sayed, N.M., Jones, K., Santos, M.R.M, Cerda, P.P., Andersson, B, Myler, P., da Silveira, J.F. & Ramirez, J.L. 2005. Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and transialidase-like gene families: the origins of T. cruzi telomeres. Gene 346:153-161.

Bartholomeu, D., El-Sayed, N.M.A. 2004. Sequencing Strategies for Parasite Genomes in Parasite Genomics Methods and Protocols – Methods in Molecular Biology (Melville, S.A, ed.). Humana Press.

El-Sayed, N.M.A., Bartholomeu, D., Ivens, A., Johnston, D.A., LoVerde, P.T. 2004. Advances in Schistosoma genomics. Trends Parasitol. 20:154-157.

LoVerde, P.T., Hirai, H., Merrick, J.M., Lee, N.H., El-Sayed, N.M.A. 2004. Schistosoma mansoni genome project: an update. Parasitol Int. 53:183-192.

Ghedin, E., Bringaud, F., Peterson, J., Myler, P., Berriman, M., Ivens, A., Andersson, B., Bontempi, E., Eisen, J., Angiuoli, S., Wanless, D., Von Arx, A., Murphy, L., Lennard, N., Salzberg, S., Adams, M.D., White, O., Hall, N., Stuart, K., Fraser, C.M., El-Sayed, N.M.A. 2004. Gene synteny and evolution of genome architecture in trypanosomatids. Mol. Biochem. Parasitol. 134:183-191.

Bringaud, F., Biteau, N., Zuiderwijk, Berriman, M., El-Sayed, N.M.A., Ghedin, E., Melville, S.A., Hall, N. and T. Baltz. 2004. The ingi and RIME non-LTR retrotransposons are not randomly distributed in the genome of Trypanosoma brucei. Mol. Biol. Evol. 21:520-528

El-Sayed,N.M.A., Ghedin,E., Song,J., MacLeod,A., Bringaud,F., Larkin,C., Wanless,D., Peterson,J., Hou,L., Taylor,S., Tweedie,A., Biteau,N., Khalak,H.G., Lin,X., Mason,T., Hannick,L., Caler,E., Blandin,G., Bartholomeu,D., Simpson,A.J., Kaul,S., Zhao,H., Pai,G., Van Aken,S., Utterback,T., Haas,B., Koo,H.L., Umayam,L., Suh,B., Gerrard,C., Leech,V., Qi,R., Zhou,S., Schwartz,D., Feldblyum,T., Salzberg,S., Tait,A., Turner,C.M., Ullu,E., White,O., Melville,S., Adams,M.D., Fraser,C.M., Donelson,J.E. 2003. The sequence and analysis of Trypanosoma brucei chromosome II. Nucleic Acids Res. 31:4856-4863.

Bartholomeu, D.C., Silva, R.A., Galvão, L.M.C., El-Sayed, N.M.A., Donelson, J.E. and S.M. Teixeira. 2002. Trypanosoma cruzi: RNA Structure and Post-Transcriptional Control of Tubulin Gene Expression Exp. Parasitol. 102:123-133.

Bringaud, F., Garcia-Perez, J.L., Heras, S.R., Ghedin, E., El-Sayed, N.M., Andersson, B., Baltz, T., Lopez, M.C. 2002. Indentification of non-autonomous non-LTR retroposons in the genome of Trypanosoma cruzi. Mol. Biochem. Parasitol. 124:73-78.

Bringaud, F., Biteau, N., Melville, S.E., Hez, S., El-Sayed, N.M., Leech, V., Berriman, M., Hall, N., Donelson, J.E. and Baltz, T. 2002. A new expressed multigene family containing a hot spot of insertion for retroelements is associated with polymorphic subtelomeric regions of Trypanosoma brucei. Euk. Cell 1:137-151.

Diehl, S., Diehl, F., El-Sayed, N.M.A., Clayton, C. and Hoheisel, J.D. 2002. Analysis of stage-specific gene expression in the bloodstream and the procyclic form of Trypanosoma brucei using a genomic DNA-microarray. Mol. Biochem. Parasitol. 123:115-123.

LaCount, D.J., El-Sayed, N.M., Kaul, S., Wanless, D., Turner, C.M.R., Donelson, J.E. 2001. Analysis of a donor VSG and its expression site in African trypanosomes. Nucleic Acids Res. 29:2012-9.

El-Sayed, N.M.A., Hegde, P., Quackenbush, J., Melville, S.E., and Donelson, J.E. 2000. The African trypanosome genome. International J. Parasitol 30:329-345.

Donelson, J.E., Hill, K.L. and El-Sayed, N.M.A. 1999. Multiple mechanisms of immune evasion by African trypanosomes. In The Trypanosome Surface (Pays, E., ed.). De Boeck & Larcier s.a., Brussels, Belgium, pp. 71-91.

Donelson, J.E., Gardner, M.J., El-Sayed, N.M. 1999. More surprises from Kinetoplastida. Proc. Natl. Acad. Sci. USA 96:2579-2581.

Clayton, C., Adams, M., Almeida, R., Baltz, T., ..., Donelson, J., El-Sayed, N., ...., Ullu, E. and Vanhamme, L. 1998. Genetic nomenclature of Trypanosoma and Leishmania. Mol. Biochem. Parasitol. 97: 221-224.

Donelson, J.E., Hill, K.L. and El-Sayed, N.M.A. 1998. Multiple mechanisms of immune evasion by African trypanosomes. Mol. Biochem. Parasitol. 91:51-66.

El-Sayed, N.M.A. and Donelson, J.E. 1997.African trypanosomes have differentially expressed genes encoding homologues of the Leishmania GP63 surface protease. J. Biol. Chem. 272: 26742-26748.

El-Sayed, N.M.A. and Donelson, J.E. 1997. A survey of the Trypanosoma brucei rhodesiense genome using shotgun sequencing. Mol. Biochem. Parasitol. 84: 167-178.

El-Sayed, N.M.A. and Donelson, J.E. 1997. Sequencing and mapping the African trypanosome genome. In Trypanosomiasis and Leishmaniasis: Biology and Control (Hide, G., Mottram, J.C., Coombs, G.H., Holmes, P.H., eds.). CAB International and the British Society for Parasitology pubs., pp 51-63.

Morgan, R.W., El-Sayed, N.M.A., Kepa, J.K., Pedram, M. and Donelson, J.E. 1996. Differential expression of the expression site-associated gene I family in African trypanosomes. J.Biol. Chem. 271: 9771-9777.

El-Sayed, N.M.A., Alarcon, C.M., Beck, J.C., Sheffield, V.C. and Donelson, J.E. 1995. cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite. Mol. Biochem. Parasitol. 73:75-90.

El-Sayed, N.M.A., Harkins, P.C., Fox, R.O., Anderson, K. and Patton, C.L. 1995. Crystallization and preliminary X-ray investigation of the recombinant Trypanosoma brucei rhodesiense calmodulin. Proteins 21:354-357.

Education

Ph.D. Yale University School of Medicine, 1993